Effect of Extraction and Centrifugation on Caffeine Levels in Urine of Robusta Coffee Drinkers Using the Method of Uv-Vis Spectrophotometry
Abstract
Caffeine is a heterocyclic alkaloid compound in the methylxanthine group that has a bitter taste that works as a mild diuretic and psychoactive stimulant. These compounds are naturally found in more than 60 types of plants, including coffee plants. Coffee is known as a drink that has a high enough caffeine content. Caffeine content in robusta coffee raw beans is higher than Arabica coffee which is only about 1.2%. The content of caffeine in the body is broken down in the liver by the enzyme cytochrome P-450 into metabolic 3-dimethylxanthin which is then excreted in the urine. Analysis of caffeine in urine was carried out quantitatively by means of UV-Vis Spectrophotometry. The analysis process is carried out by extraction and centrifugation. Based on this study, a study was conducted to determine the differences in extraction and centrifugation methods on caffeine levels in the urine of robusta coffee drinkers using the UV-Vis spectrophotometric method. The results were analyzed using the Paired T-Test through SPSS. The results showed that there was significant difference in the levels of caffeine extracted and centrifuged in the urine of robusta coffee drinkers using the UV-Vis Spectrophotometry method. The caffeine content in the urine sample was obtained with the lowest extraction at 29.60 ppm and the highest caffeine content at 50.18 ppm. Meanwhile, in urine samples by centrifugation, the lowest caffeine content was 35.71 ppm and the highest caffeine content was 50.45 ppm.
References
Abraham. 2010. Penuntun Praktikum Kimia Organik II.UNHALU : Kendari
Alsabri, S.G, BscPharm, MS., Mari, W.O., MD, MS., Younes, S., MD, MS., Alsadawi, M.A., MD, & Oroszi, T.L., MS, EdD. 2018. Kinetic and Dynamic Description of Caffeine. Journal of Caffeine and Adenosine Research. 8(1): 3-9
Aprilia, F. A., Ayuliansari, Y.P, T. Azis, M. Camelina, W., dan Putra, M. (2018). Analisis Kandungan Kafein dalam Kopi Tradisional Gayo dan Kopi Lombok Menggunakan HPLC dan Spektrofotometri UV-Vis. Biotika. 16 (2) : 38-39.
Arnaud MJ (2011) Farmakokinetik dan metabolisme methylxanthines alami pada hewan dan manusia, di Methylxanthines. Buku Pegangan Farmakologi Eksperimental, vol 200, hlm 33–91, Springer, Heidelberg.
Arwangga, A. F., Ida, A. R. A. A., dan Wayan, I. S. 2016. Analisis Kandungan Kafein Pada Kopi Di Desa Sesaot Narmada Menggunakan Spektrofotometri Uv-Vis. Jurnal Kimia. 10(1): 110-114.
Cornelis, C. M. (2019). The Impact of Caffeine and Coffee on Human Health. Nutrients Journal, 11, 416.
Departemen Kesehatan RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Diktorat Jendral POM Depkde RI.
Ennis, D., 2014. The Effect of Caffeine on Healt: The Benefits Outweigh the Risk. Academic Journal.
Farida, A., Evi, R. R., dan Kumoro, A. C., 2013. Penurunan Kadar Kafein dan Asam Total pada Biji Kopi Robusta Menggunakan Teknologi Fermentasi Anaerob Fakultatif dengan Mikroba Nopkor MZ-15. Jurnal Teknologi Kimia dan Industri. 2(2): 70–75.
Fitri, N. S., 2008. Pengaruh Berat dan Waktu Penyeduhan terhadap Kadar Kafein dari Bubuk Teh. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan.
Ibrahim, M. S. D., dan Sri, H. 2017. Peningkatan Induksi Kalus Embriogenik Dan Konversi Embrio Somatik Kopi Robusta Klon Bp 308. Journal of Industrial and Beverage Crops. 4(3): 121-132.
Kumar, T.H., Mounika,P.,Likhita,S.S., Aswini, S., Kavya, T., Sudandhini, T., & Sree, A.V. 2019. Aplication of Stability Indicating UV Spectrophotometric Method for Estimation of Caffeine in Gel Formulation, Plasma and Urine. International Journal of Pharmaceutical Sciences and Research. 10(12): 5481-5488
Latunra, A.A., Johannes, E., Mulihardianti, B., & Sumule, O. 2021. Analisis Kandungan Kafein Kopi (Coffea Arabica) Pada Tingkat Kematangan Berbeda Menggunakan Spektrofotometer UV-Vis. Jurnal Ilmu Alam dan Lingkungan. 12(1):45-50
Lopez,S.M., Sarria, B., Baeza, G., Mateos, R., Clemente, L.B. 2014. Pharmacokinetic of Caffeine and its Metabolites in Plasma and Urine After Consuming a Soluble Green/Roasted Coffe Blend by Healthy Subjects. Food Research International. 64 : 125-133
Maramis, R. K., Citraningtyas, G., Wehantouw F. (2013). Analisis Kafein dalam Kopi Bubuk di Kota Manado Menggunakan Spektrofotometri UV-Vis. Pharmacon Jurnal Ilmiah Farmasi, Vol 2, No.4 : 122-128.
Muhibatul, 2014. Analisis Kandungan Kafein Pada Kopi. Skripsi. Fakultas Ilmu Tarbiyah dan Keguruan. IAIN. Semarang.
Özpalas, B. dan Özer, E. A. (2017). Effects of Caffeine on Human Health. Nev?ehir Billim ve Teknoloji Dergisi Cilt, 6: 297-305.
Rahmawati, M. A., dan Kiki, F. 2018. Karakterisasi Sensori Kopi Robusta Dampit: Kajian Pustaka. Jurnal Pangan dan Agroindustri. 6(1): 75-79.
Siahaan, M.A. 2019. Korelasi Antara Jumlah Kafein yang Dikonsumsi dalam Air Kopi dengan Jumlah Kafein yang Diekskresikan dalam Urine. Majalah Ilmiah Methoda. 9(2): 86-88
Wachamo, H. L. (2017). Review on Health Benefit and Risk of Caffeine Consumption. Medical & Aromatic Plants Journal, 11:416.
Wanyika, H.N., Gatebe, E.G., Gitu, L.M., Ngumba, E.K., & Maritim, C.W. 2010. Determination of caffeine content of tea and instant coffe brands found in the Kenyan market. African Journal of Food Science. 4(6): 353-358
Wibawa, Indra. 2012. Ekstraksi Cair-Cair. Lampung: Teknik Kimia Universitas LampungAbraham. 2010. Penuntun Praktikum Kimia Organik II.UNHALU : Kendari
Alsabri, S.G, BscPharm, MS., Mari, W.O., MD, MS., Younes, S., MD, MS., Alsadawi, M.A., MD, & Oroszi, T.L., MS, EdD. 2018. Kinetic and Dynamic Description of Caffeine. Journal of Caffeine and Adenosine Research. 8(1): 3-9
Aprilia, F. A., Ayuliansari, Y.P, T. Azis, M. Camelina, W., dan Putra, M. (2018). Analisis Kandungan Kafein dalam Kopi Tradisional Gayo dan Kopi Lombok Menggunakan HPLC dan Spektrofotometri UV-Vis. Biotika. 16 (2) : 38-39.
Arnaud MJ (2011) Farmakokinetik dan metabolisme methylxanthines alami pada hewan dan manusia, di Methylxanthines. Buku Pegangan Farmakologi Eksperimental, vol 200, hlm 33–91, Springer, Heidelberg.
Arwangga, A. F., Ida, A. R. A. A., dan Wayan, I. S. 2016. Analisis Kandungan Kafein Pada Kopi Di Desa Sesaot Narmada Menggunakan Spektrofotometri Uv-Vis. Jurnal Kimia. 10(1): 110-114.
Cornelis, C. M. (2019). The Impact of Caffeine and Coffee on Human Health. Nutrients Journal, 11, 416.
Departemen Kesehatan RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Diktorat Jendral POM Depkde RI.
Ennis, D., 2014. The Effect of Caffeine on Healt: The Benefits Outweigh the Risk. Academic Journal.
Farida, A., Evi, R. R., dan Kumoro, A. C., 2013. Penurunan Kadar Kafein dan Asam Total pada Biji Kopi Robusta Menggunakan Teknologi Fermentasi Anaerob Fakultatif dengan Mikroba Nopkor MZ-15. Jurnal Teknologi Kimia dan Industri. 2(2): 70–75.
Fitri, N. S., 2008. Pengaruh Berat dan Waktu Penyeduhan terhadap Kadar Kafein dari Bubuk Teh. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan.
Ibrahim, M. S. D., dan Sri, H. 2017. Peningkatan Induksi Kalus Embriogenik Dan Konversi Embrio Somatik Kopi Robusta Klon Bp 308. Journal of Industrial and Beverage Crops. 4(3): 121-132.
Kumar, T.H., Mounika,P.,Likhita,S.S., Aswini, S., Kavya, T., Sudandhini, T., & Sree, A.V. 2019. Aplication of Stability Indicating UV Spectrophotometric Method for Estimation of Caffeine in Gel Formulation, Plasma and Urine. International Journal of Pharmaceutical Sciences and Research. 10(12): 5481-5488
Latunra, A.A., Johannes, E., Mulihardianti, B., & Sumule, O. 2021. Analisis Kandungan Kafein Kopi (Coffea Arabica) Pada Tingkat Kematangan Berbeda Menggunakan Spektrofotometer UV-Vis. Jurnal Ilmu Alam dan Lingkungan. 12(1):45-50
Lopez,S.M., Sarria, B., Baeza, G., Mateos, R., Clemente, L.B. 2014. Pharmacokinetic of Caffeine and its Metabolites in Plasma and Urine After Consuming a Soluble Green/Roasted Coffe Blend by Healthy Subjects. Food Research International. 64 : 125-133
Maramis, R. K., Citraningtyas, G., Wehantouw F. (2013). Analisis Kafein dalam Kopi Bubuk di Kota Manado Menggunakan Spektrofotometri UV-Vis. Pharmacon Jurnal Ilmiah Farmasi, Vol 2, No.4 : 122-128.
Muhibatul, 2014. Analisis Kandungan Kafein Pada Kopi. Skripsi. Fakultas Ilmu Tarbiyah dan Keguruan. IAIN. Semarang.
Özpalas, B. dan Özer, E. A. (2017). Effects of Caffeine on Human Health. Nev?ehir Billim ve Teknoloji Dergisi Cilt, 6: 297-305.
Rahmawati, M. A., dan Kiki, F. 2018. Karakterisasi Sensori Kopi Robusta Dampit: Kajian Pustaka. Jurnal Pangan dan Agroindustri. 6(1): 75-79.
Siahaan, M.A. 2019. Korelasi Antara Jumlah Kafein yang Dikonsumsi dalam Air Kopi dengan Jumlah Kafein yang Diekskresikan dalam Urine. Majalah Ilmiah Methoda. 9(2): 86-88
Wachamo, H. L. (2017). Review on Health Benefit and Risk of Caffeine Consumption. Medical & Aromatic Plants Journal, 11:416.
Wanyika, H.N., Gatebe, E.G., Gitu, L.M., Ngumba, E.K., & Maritim, C.W. 2010. Determination of caffeine content of tea and instant coffe brands found in the Kenyan market. African Journal of Food Science. 4(6): 353-358
Wibawa, Indra. 2012. Ekstraksi Cair-Cair. Lampung: Teknik Kimia Universitas Lampung
Wilson, C. (2018). The Clinical Toxicology of Caffeine: A Review and Case Study. Elsivier (Toxicology Reports), 5: 1140-1152.Wilson, C. (2018). The Clinical Toxicology of Caffeine: A Review and Case Study. Elsivier (Toxicology Reports), 5: 1140-1152.
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